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Systematic Optimization of Urine DNA Isolation for Microbiome Sequencing
2023-08-08
Systematic Optimization of Urine DNA Isolation for Microbiome Sequencing
Systematic Optimization of Urine DNA Isolation for Microbiome Sequencing Recent advances in DNA sequencing technology have enabled the characterization of microbial communities in different parts of the human body using culture-independent techniques. Studies using these methods have demonstrated that the urinary tract contains diverse microbial populations, termed the uri...
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Enhance DNA Extraction from Urine Using Tris-EDTA Treatment
2023-08-08
Enhance DNA Extraction from Urine Using Tris-EDTA Treatment
Enhance DNA Extraction from Urine Using Tris-EDTA Treatment Urine samples are commonly used for molecular detection of bacteria that colonize the genital tract. However, low concentrations of bacteria and the presence of PCR inhibitors like urea and crystals in urine can hamper efficient DNA extraction and subsequent molecular testing. A study in 2019 aimed to develop an o...
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UTI Stops DNA Degradation in Frozen Forensic Urine Samples
2023-08-08
UTI Stops DNA Degradation in Frozen Forensic Urine Samples
UTI Stops DNA Degradation in Frozen Forensic Urine Samples Urine represents an abundant and easily accessible biological material that could provide an alternative source of DNA for forensic genotyping and identification. However, DNA from urinary epithelial cells and cell-free DNA tends to degrade rapidly from enzymatic, chemical, and microbial damage during storage. This...
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How Hydrophobicity Makes THC Tricky to Handle Analytically
2023-08-02
How Hydrophobicity Makes THC Tricky to Handle Analytically
How Hydrophobicity Makes THC Tricky to Handle Analytically Why is THC so Extremely Hydrophobic? THC is highly hydrophobic, with an octanol-water partition coefficient (log Kow) as high as 6.4. This extreme hydrophobicity arises from THC's chemical structure containing effective alkyl substituents and aromatic ring systems. The numerous nonpolar C-H bonds in THC stre...
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Saliva Collector Benefits for Roadside Drug Testing
2023-08-02
Saliva Collector Benefits for Roadside Drug Testing
Saliva Collector Benefits for Roadside Drug Testing Drug testing has advanced significantly in recent years, with increasing use of alternative specimens to blood or urine to detect drug exposure. Oral fluid has emerged as a promising non-invasive specimen for drug testing, particularly to identify recent drug use. Technological developments have also enabled on-site oral ...
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Diagnostic Odysseys: The Critical Role of Viral Transport Media
2023-08-02
Diagnostic Odysseys: The Critical Role of Viral Transport Media
Diagnostic Odysseys: The Critical Role of Viral Transport Media The successful diagnosis of viral infections relies heavily on proper collection, transport, and timely inoculation of clinical specimens containing infectious viral particles. Different viruses exhibit wide variability in composition, structure, morphology, stability and susceptibility to inactivation. Optimi...
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Collecting Poop? You Need Cary-Blair, The Microbiome Saver
2023-07-28
Collecting Poop? You Need Cary-Blair, The Microbiome Saver
Collecting Poop? You Need Cary-Blair, The Microbiome Saver The human gut microbiome plays an important role in health and disease. Advances in sequencing have enabled detailed characterization of the gut microbiota composition. However, sample collection and storage can significantly impact the bacterial composition. This highlights the need to optimize fecal sampling prot...
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The Hidden Truth of Group B Streptococci Survival in Amies Transport Medium
2023-07-28
The Hidden Truth of Group B Streptococci Survival in Amies Transport Medium
The Hidden Truth of Group B Streptococci Survival in Amies Transport Medium Recently, a research team led by Annaliesa S. Anderson from the American pharmaceutical company Pfizer, explored the potential of maternal immunization with an infant Group B Streptococcus vaccine. The findings of this study were published in the New England Journal of Medicine on July 19, 2023. ...
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Why Charcoal is Crucial in Amies Transport Medium for Gonorrhea Detection
2023-07-27
Why Charcoal is Crucial in Amies Transport Medium for Gonorrhea Detection
Why Charcoal is Crucial in Amies Transport Medium for Gonorrhea Detection Gonorrhea is a major public health issue worldwide. The bacteria that causes gonorrhea, Neisseria gonorrhoeae, has developed resistance to nearly all antimicrobials used for treatment. Being able to obtain live N. gonorrhoeae isolates through culture is essential for monitoring antimicrobial resistan...
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Mantacc in Spotlight: An Exciting First Day at ADLM 2023
2023-07-26
Mantacc in Spotlight: An Exciting First Day at ADLM 2023
Mantacc in Spotlight: An Exciting First Day at ADLM 2023 From July 25th to 27th, 2023, the 75th AACC Annual Scientific Meeting & Clinical Lab Expo will be held grandly at the Anaheim Convention Center in California, USA. This grand event will bring together 941 exhibiting companies from more than 100 countries and regions around the world, becoming an international eve...
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Ethanol vs Isopropyl Alcohol: Which Works Better With CHG Applicator For Skin Preparation?
2023-07-19
Ethanol vs Isopropyl Alcohol: Which Works Better With CHG Applicator For Skin Preparation?
Ethanol vs Isopropyl Alcohol: Which Works Better With CHG Applicator For Skin Preparation? Chlorhexidine gluconate (CHG) antiseptic applicators are commonly used to disinfect the skin prior to medical procedures like placing IV lines or drawing blood. Traditionally, these applicators have contained a combination of CHG and isopropyl alcohol (also known as isopropanol or IP...
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Everything You Need To Know About Cervical Specimen Collection
2023-07-17
Everything You Need To Know About Cervical Specimen Collection
Everything You Need To Know About Cervical Specimen Collection Cervical cancer screening through tests like the Pap smear and HPV test are vital prevention tools all women should utilize. Regular screening allows for the detection of abnormal cervical cells and HPV infection before they potentially develop into cancer. While screening procedures have improved dramaticall...
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