Best Time to Collect Saliva for DNA Test
By following the recommended best practices for saliva collection, you can increase the likelihood of obtaining high-quality DNA samples for accurate and reliable testing results.
When is the Ideal Time?
The ideal time to collect saliva for DNA testing is typically when you have an empty stomach. It is recommended to wait at least one hour after eating a meal before collecting your saliva sample. This helps ensure that there are no food particles or other contaminants present in your saliva, which could interfere with the DNA extraction process.
How to Prepare for Saliva Collection?
Before collecting your saliva sample, it is advisable to rinse your mouth thoroughly with water to remove any lingering food particles or residue. Allow at least ten minutes to pass after rinsing before collecting your saliva. This waiting period ensures that your saliva is not diluted and contains an optimal concentration of DNA-containing cells.
Proper Collection Techniques
There are two primary methods for collecting saliva samples for DNA testing:
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1. Passive Drool Method: In this method, you allow saliva to naturally pool in your mouth and then gently drool it into a collection tube or container. This method collects whole saliva, which contains cells from the inner linings of your mouth as well as white blood cells.
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2. Oral Swab Method: This method involves using a specialized swab to collect saliva from the inside of your cheeks and gums. The swab is then placed in a collection container or tube.
Regardless of the method used, it is crucial to follow proper handling procedures to protect the integrity of the DNA sample. This may include using clean, disposable gloves and storing the collected sample on ice or freezing it immediately after collection.
Considerations for Optimal DNA Yield
To ensure an optimal DNA yield from your saliva sample, it is essential to follow any specific instructions provided by the testing laboratory or kit manufacturer. Some factors that can affect the quality and quantity of DNA extracted include:
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Hydration level: Being well-hydrated can help increase saliva production and improve DNA yield.
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Collection time: Collecting saliva in the morning, before consuming any food or beverages, may provide the best results.
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Oral hygiene: Brushing your teeth or using mouthwash immediately before collection can potentially remove some DNA-containing cells from your mouth.
Click to View → Mantacc TY-001 Integrated Saliva Collection Kit, Mantacc 93052 Saliva Collection Stick
The Mantacc saliva collection devices can be used for a variety of study types such as RNA collection, oral microbiome collection and saliva DNA collection. In addition to this, the saliva samples can then be used for many downstream processes including genotyping, sequencing, SNP and diagnostic use, as well as more recently being utilized for testing purposes such as for the Sars-covid-19 virus.
