【Expected Cultivation Experiment】
The bacterial strains used for quality control of Stuart Transport Medium include:
Streptococcus pyogenes (ATCC 19615):
- Aerobic gram-positive coccus
- Quality control requirement: Able to be isolated again after aerobic subculture for 24 hours
Bacteroides fragilis (ATCC 25285):
- Obligate anaerobe
- Quality control requirement: Able to be isolated again after aerobic subculture for 24 hours
Pseudomonas aeruginosa (ATCC 27853):
- Aerobic gram-negative bacillus with weak oxygen requirement
- Quality control requirement: Able to be isolated again after aerobic subculture for 24 hours
The rationale for choosing these 3 strains is to represent gram-positive cocci, gram-negative bacilli, and anaerobes with different oxygen requirements, covering the typical application range of the medium comprehensively as appropriate quality control strains.
【Precautions】
1. All clinical specimens should be treated as potentially biohazardous. Use appropriate personal protective equipment and follow laboratory and biosafety protocols when handling.
2. This product is for use by properly trained and qualified personnel only. Read and follow the instructions carefully.
3. Refer to CDC Laboratory Biosafety Manual for related regulations.
4. Do not use expired products.
5. Do not use products with damaged packaging.
6. Avoid excessive force when collecting samples to prevent breaking the swab.
7. Use sterile forceps to remove swab from bottle.
8. Sterilize after use and dispose of biohazardous waste according to regulations.
9. Do not ingest the modified liquid Stuart transport medium.
10. Avoid freezing and high temperature conditions.