【Expected Cultivation Experiment】
Separately incubate inocula for 18-24 hours on Soybean Casein Digest Agar (DM247) and observe cultural characteristics. Use inoculum amounts of 50-100 CFU.
Recommended Quality Control Strains and Results:
Amies with charcoal culture medium:
1. Enterococcus faecalis (ATCC 25285), aerobic, 25-30°C 24 hours, good recovery in subsequent cultivation.
2. Haemophilus influenzae (ATCC 10211), aerobic, 25-30°C 24 hours, good recovery in subsequent cultivation.
3. Neisseria gonorrhoeae (ATCC 43069), aerobic, 25-30°C 24 hours, good recovery in subsequent cultivation.
4. Staphylococcus aureus (ATCC 19615), aerobic, 25-30°C 24 hours, good recovery in subsequent cultivation.
Amies without charcoal culture medium:
1. Enterococcus faecalis (ATCC 25285), aerobic, 25-30°C 24 hours, good recovery in subsequent cultivation.
2. Staphylococcus aureus (ATCC 19615), aerobic, 25-30°C 24 hours, good recovery in subsequent cultivation.
Verifying with these recommended quality control strains can confirm the Amies culture media meet the required performance standards.
【Precautions】
1. All clinical specimens should be treated as potentially biohazardous. Use appropriate personal protective equipment and follow laboratory and biosafety protocols when handling.
2. This product is for use by properly trained and qualified personnel only. Read and follow the instructions carefully.
3. Refer to CDC Laboratory Biosafety Manual for related regulations.
4. Do not use expired products.
5. Do not use products with damaged packaging.
6. Avoid excessive force when collecting samples to prevent breaking the swab.
7. Use sterile forceps to remove swab from bottle.
8. Sterilize after use and dispose of biohazardous waste according to regulations.
9. Do not ingest the modified liquid Amies transport medium.
10. Avoid freezing and high temperature conditions.