How Has Specimen Collection Changed Over the Years?
How Has Specimen Collection Changed Over the Years?

How Has Specimen Collection Changed Over the Years?

Tubes of blood sample for testing. Medical equipment

Specimen collection devices like swabs and blood collection tubes play a critical role in healthcare and research. But how did we get to where we are today? What were the key innovations that shaped specimen collection as we know it?

In the early days of modern medicine, specimen collection was difficult and imprecise. Blood would be collected in simple glass tubes with etched or painted lines to indicate the amount needed. The tubes contained additives like EDTA or citrate to prevent clotting. After collection, black rubber stoppers sealed the tubes for transport to the lab. Needles were washed and sterilized between patients to control infection.

Things started to change in the 1940s and 50s. In 1947, Joseph Kleiner invented the Evacutainer, a glass vacuum tube for drawing blood. The vacuum pressure ensured a correct fill volume, and the disposable nature improved sanitation. This was later trademarked as the Vacutainer system by Becton, Dickinson and Company.

In the 1960s, Dr. R.D. Stuart developed an early transport medium for swabs, allowing them to maintain organism viability for lab analysis. His Stuart's medium contained ingredients like sodium thioglycolate, glycerophosphate, calcium chloride and methylene blue. Later innovations by Dr. Amies improved on this formula.

These transport media systems enabled the sterile collection and transport of specimens like wound cultures or vaginal swabs. Paired with a swab, the medium kept organisms alive for lab analysis.

Initially, cotton was used for swab tips. But cotton fibers contain fatty acids that can inhibit some organisms. So companies developed synthetic alternatives like rayon and polyester. Dacron polyester offered the advantage of not absorbing specimens, but rather collecting them on the surface for easy release to a test device or culture plate.

In the 1990s, new materials like polyurethane foam were introduced for swabs. Foam offered advantages for collecting and transporting specimens dry, without the need for an ampouled transport medium. This enabled self-contained collection and transport systems ideal for point-of-care testing.

In the 2000s, flocked swabs were developed using technologies from the cosmetics industry. Instead of wrapped fibers, flocking attaches short perpendicular fibers to the tip using an electrostatic process. This creates a high surface area ideal for collecting specimens. Flocked swabs are now considered optimal for microbial sampling and molecular collection applications.

Blood collection also continued to advance. In the early 1990s, plastic vacuum tubes like the Vacutainer PLUS were introduced. Plastic offered benefits like easier disposal and reduced breakage.

In the 2000s, dried blood spot sampling on filter paper cards created a simpler way to collect small samples in the field. But variations in absorption remained a challenge. Newer volumetric absorptive microsampling devices overcame issues like hematocrit bias that limited dried blood spots.

Today, regulatory agencies like the FDA and CLSI enforce strict standards for design and manufacture of specimen collection devices. This ensures that modern swabs, transport media, and blood collection tools deliver the precision and accuracy needed for modern medicine and research.

The key innovations enabling current specimen collection methods were:

  • - Vacuum tube blood collection
  • - Swabs paired with transport media
  • - Synthetic swab materials like polyester and foam
  • - Flocked swab technology
  • - Plastic vacuum tubes
  • - Dried blood spot sampling
  • - Volumetric microsampling for precision

What does the future hold? Emerging frontiers like bioprinting, microfluidics, and telemedicine will likely shape the next generation of devices. But our current reliable tools for blood, microbial, and molecular sampling all owe a debt to the pioneering work of inventors over the past 75 years. Specimen collection may not seem exciting, but constant improvement in this critical pre-analytical step enables advances throughout healthcare and the life sciences.

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